Supplemental Assay Methods (SAMs)
Last Modified: October 08, 2024
| ID | Title | Date |
|---|---|---|
| 101 | Titration of Bovine Viral Diarrhea Virus in Vaccines [Withdrawn] | -- |
| 102 | Titration of Parainfluenza-3 Virus in Vaccines [Withdrawn] | -- |
| 104 (146.07 KB) | Isolation of Parainfluenza-3 Virus from Nasal Excretions | October 10, 2014 |
| 105 | Titration of Bovine Rhinotracheitis Virus in Vaccines [Withdrawn] | -- |
| 106 (181.85 KB) | Titration of Neutralizing Antibody (Constant Virus - Varying Serum Method) | October 16, 2014 |
| 107 (164.92 KB) | Titration of Neutralizing Antibody (Constant Serum - Varying Virus Method) | October 16, 2014 |
| 108 (235.28 KB) | Detection of Extraneous Bovine Viral Diarrhea Virus in Modified-Live Vaccines | July 29, 2023 |
| 109 (295.5 KB) | Titration of Bovine Rhinotracheitis Neutralizing Antibody (Constant Virus - Varying Serum Method) | October 16, 2014 |
| 110 (257.13 KB) | Titration of Eastern, Western, and Venezuelan Equine Encephalomyelitis Virus Neutralizing Antibodies | April 05, 2016 |
| 111 | Titration of Infectious Bovine Rhinotracheitis Virus Neutralizing Antibody (Constant Virus - Varying Serum Method) [Withdrawn] | -- |
| 112 (255.58 KB) | Titration of Bovine Viral Diarrhea Neutralizing Antibody (Constant Virus - Varying Serum Method) | October 16, 2014 |
| 113 (278.25 KB) | Titration of Parainfluenza-3 Neutralizing Antibody (Constant Virus - Varying Serum Method) | May 12, 2017 |
| 114 (196.51 KB) | Titration of Porcine Transmissible Gastroenteritis Virus | July 31, 2023 |
| 115 (183.31 KB) | Titration of Bovine Viral Diarrhea Neutralizing Antibody (Constant Serum - Varying Virus Method) | October 16, 2014 |
| 116 (158.05 KB) | Phenotypic Examination of Pseudorabies Virus for Thymidine Kinase Activity by a Plaque Selection Method | October 16, 2014 |
| 117 (254.5 KB) | Titration of Pseudorabies Virus Neutralizing Antibody (Constant Virus - Varying Serum Methods) | October 16, 2014 |
| 118 (130.5 KB) | Titration of Pseudorabies Virus in Vaccines | August 7, 2023 |
| 119 (172.12 KB) | Titration of Pseudorabies Virus Neutralizing Antibody (Constant Virus - Varying Serum Method) | August 7, 2023 |
| 120 (220.4 KB) | In Vitro Potency Assay of Bovine Respiratory Viruses in Killed Vaccines | September 15, 2023 |
| 121 (148.42 KB) | Titration of Porcine Rotavirus in Modified-live Vaccines | October 21, 2014 |
| 122 (216.34 KB) | Titration of Porcine Rotavirus Antibody (Constant Virus-Varying Serum Method) | October 21, 2014 |
| 123 (171.34 KB) | Pseudorabies Virus Challenge Test in Swine | October 21, 2014 |
| 124 (226.66 KB) | Conducting the Hemagglutination Inhibition Assay for Equine Influenza Antibody | October 23, 2014 |
| 126 (217.06 KB) | Titration of Bovine Rotavirus in Vaccines (Constant Virus - Varying Serum Method) | August 9, 2023 |
| ID | Title | Date |
|---|---|---|
| 200 (239.54 KB) | Potency Testing Products Containing Clostridium chauvoei Antigen | March 23, 2024 |
| 201 (163.69 KB) | Potency Testing Clostridium perfringens Type C Beta Antigen | August 24, 2023 |
| 202 (226.31 KB) | Potency Testing Clostridium perfringens Type C Beta Antitoxins | August 24, 2023 |
| 203 (166.81 KB) | Potency Testing Clostridium perfringens Type D Epsilon Antigen | August 24, 2023 |
| 204 (229.8 KB) | Potency Testing Clostridium perfringens Type D Epsilon Antitoxins | September 7, 2023 |
| 206 (322.24 KB) | Potency Testing Tetanus Antitoxins | March 15, 2024 |
| 207 (275.62 KB) | Potency Testing Clostridium novyi Type B Alpha Antigen | August 10, 2023 |
| 209 (157.03 KB) | Potency Testing Products Containing Clostridium haemolyticum Antigen | December 13, 2017 |
| 212 (166.54 KB) | Potency Testing Clostridium sordellii Antigen | August 10, 2023 |
| 213 (158.45 KB) | Potency Testing of Clostridium botulinum Type C Bacterin-Toxoids | June 17, 2022 |
| 214 (155.75 KB) | Immunogenicity and Spore Count Testing of Anthrax Spore Vaccine-Nonencapsulated | June 12, 2022 |
| 217 (228.44 KB) | Potency Testing of Tetanus Toxoid by ELISA | June 17, 2022 |
| ID | Title | Date |
|---|---|---|
300 (185.81 KB) | Titration of Neutralizing Antibody - Canine Hepatitis | August 22, 2023 |
| 301 (211.26 KB) | Titration of Neutralizing Antibody - Canine Distemper | August 22, 2023 |
| 302 (212.99 KB) | Titration of Canine Distemper Virus in Primary Cell Culture | October 29, 2014 |
| 303 (237.39 KB) | Titration of Distemper Virus in Embryonated Chicken Eggs | August 22, 2023 |
| 304 (205.85 KB) | Titration of Infectious Canine Hepatitis Virus in Primary Canine Kidney Cell Culture | October 30, 2014 |
| 305 (208.69 KB) | Titration of Feline Panleukopenia Virus in Cell Culture | October 30, 2014 |
| 306 (179.35 KB) | Titration of Feline Calicivirus in Cell Culture | October 30, 2014 |
| 307 (155.38 KB) | Titration of Feline Rhinotracheitis Virus in Cell Culture | October 30, 2014 |
| 308 (176.85 KB) | Potency Testing of Inactivated Rabies Vaccine in Mice Using the National Institutes of Health Test | March 31, 2015 |
| 309 (215.01 KB) | Titration of Canine Parainfluenza Virus in Vero Cell Culture | October 30, 2014 |
| 310 | Scoring Feline Calicivirus in Cats Following Challenge [Withdrawn] | -- |
| 311 | Scoring Feline Rhinotracheitis Virus in Cats Following Challenge [Withdrawn] | -- |
| 312 (124.13 KB) | Detection of Extraneous Cytopathogenic Agents in Master Seed Virus | October 30, 2014 |
| 313 (272.38 KB) | Detection of Extraneous Hemadsorbing Agents in Master Seed Virus | August 23, 2023 |
| 314 (150.1 KB) | Detection of Extraneous Viruses by the Fluorescent Antibody Test | November 13, 2014 |
| 315 (181 KB) | Detection of Serum Neutralizing Antibodies to Rabies Virus using the Rapid Fluorescent Focus Inhibition Test (RFFIT) | November 13, 2014 |
| 316 (205.34 KB) | Titration of Canine Parvovirus in Cell Culture | November 14, 2014 |
| 317 (200.82 KB) | Titration of Canine Adenovirus in Canine Kidney Cell Culture | November 13, 2014 |
| 318 | Estimating Relative Potency by the Automated Selection of Line Segments in RelPot 4.0 Software [Withdrawn] | -- |
| 319 (307 KB) | Titration of Chlamydophila felis (formerly Feline Chlamydia psittaci) in Cell Culture | November 13, 2014 |
| 320 (144.95 KB) | Titration of Feline Chlamydia psittaci in Embryonated Chicken Eggs | November 13, 2020 |
| 321 (164.72 KB) | Quantitating the GP70 Antigen of Feline Leukemia Virus in Veterinary Vaccines | January 9, 2015 |
| 322 (213.13 KB) | Determination of the Specific Viral Antigen Content in Inactivated Canine Coronavirus Vaccines | January 9, 2015 |
| 323 (205.35 KB) | Titration of Canine Distemper Virus in Vero Cell Culture | November 20, 2020 |
| ID | Title | Date |
|---|---|---|
| 405 (350.25 KB) | Supplemental Assay Method for Detecting Lymphoid Leukosis Biocontamination by the COFAL Test | August 25, 2023 |
| 406 (206.68 KB) | Supplemental Assay Method for Titration of Monovalent, Cell Associated Marek’s Disease Vaccines of Serotypes 1, 2, or 3 | September 14, 2024 |
| 407 (182.25 KB) | Supplemental Assay Method for Titration of Marek’s Serotype 3 (Herpesvirus of Turkeys Strain FC-126), Lyophilized | August 29, 2023 |
| 408 (167.15 KB) | Supplemental Assay Method for Titrating Tissue Culture Adapted Vaccine Strains of Infectious Bursal Disease Virus | August 29, 2023 |
| 409 (122.5 KB) | Titrating the Fractions of Combination Avian Encephalomyelitis/Pox Vaccine | November 28, 2014 |
| 411 (157.13 KB) | Titration of Newcastle Disease Vaccine, Infectious Bronchitis Vaccine, and Combination Newcastle Disease-Infectious Bronchitis Vaccine in Chicken Embryos | January 10, 2018 |
| ID | Title | Date |
|---|---|---|
| 500 (1.01 MB) | Determination of Phenol in Veterinary Biologics (Pullorum antigen, Mycoplasma synoviae antigen, and Mycoplasma gallisepticum antigen) | June 16, 2022 |
| 502 (1.69 MB) | Determination of Residual Moisture in Veterinary Biologics Products | June 17, 2022 |
| 504 (882.54 KB) | Manual Determination of Protein Content of Veterinary Biologics (Biuret) | June 17, 2022 |
| 509 | Mikrozone Electrophoretic Separation of Blood Fractions Used in Veterinary Biologics [Withdrawn] | -- |
| 512 (162.32 KB) | Determination of Formaldehyde in Veterinary Biologics (Ferric Chloride Test) | April 5, 2022 |
| 513 (416.58 KB) | Determination of Protein and Phenol in PPD Bovis Tuberculin (Purified Protein Derivative Produced from Cultures of Mycobacterium bovis (Strain AN-5)) | January 5, 2024 |
| 514 (433.8 KB) | Determination of Hydrogen Ion Concentration, Total Nitrogen, Phenol and Clarity in Intradermic Tuberculin (Filtrate Produced From Cultures of Pn, C, and Dt Strains of Mycobacterium tuberculosis) | January 5, 2024 |
| ID | Title | Date |
|---|---|---|
| 600 (224.18 KB) | Purity, Potency, and Dissociation of Brucella abortus Vaccine, Strain 19 | January 3, 2022 |
| 601 | Potency Testing of Erysipelas Antiserum in Mice [Withdrawn] | -- |
| 602 (46.36 KB) | Evaluation of Salmonella Pullorum Antigens | January 28, 2010 |
| 603 (925.05 KB) | Potency Testing of Fowl Cholera (Pasteurella multocida) Bacterins, Type 3 | June 17, 2022 |
| 604 (1.09 MB) | Evaluation of Koch's Old Tuberculin | June 17, 2022 |
| 605 | Potency Testing of Erysipelas Bacterins in Swine [Withdrawn] | -- |
| 606 | Potency Testing of Erysipelas Vaccines in Swine [Withdrawn] | -- |
| 607 (929.34 KB) | Potency Testing of Fowl Cholera (Pasteurella multocida) Bacterins, Type 1 | June 17, 2022 |
| 608 (257.19 KB) | Potency Assay of Leptospira interrogans serogroup pomona Bacterins | December 23, 2022 |
| 609 (254.96 KB) | Potency Assay of Leptospira interrogans serogroup canicola Bacterins | April 19, 2022 |
| 610 (242.52 KB) | Potency Assay of Leptospira interrogans serogroup icterohaemorrhagiae Bacterins | December 23, 2022 |
| 611 (221.17 KB) | Potency Testing of Erysipelas Bacterins in Mice | December 15, 2017 |
| 612 (126.2 KB) | Bacterial Plate Count of Erysipelothrix rhusiopathiae Vaccines | February 05, 2015 |
| 613 (906.22 KB) | In vitro Potency Testing of Erysipelothrix rhusiopathiae Bacterins | June 17, 2022 |
| 617 (225.91 KB) | Potency Assay Leptospira kirschneri serogroup grippotyphosa Bacterins | April 26, 2022 |
| 620 (256.57 KB) | Potency Testing of Enterotoxigenic (K99 Pilus) Escherichia coli Bacterins | May 26, 2023 |
| 621 (245.17 KB) | Potency Testing of Enterotoxigenic (K88 Pilus) Escherichia coli Bacterins | May 26, 2023 |
| 622 (246.24 KB) | Potency Testing of Enterotoxigenic (987P Pilus) Escherichia coli Bacterins | May 26, 2023 |
| 623 (248.28 KB) | Potency Testing Enterotoxigenic (F41 Pilus) Escherichia coli Bacterins | May 26, 2023 |
| 624 (215.31 KB) | In vitro Potency Testing of Leptospira interrogans serogroup pomona Bacterins | May 28, 2023 |
| 625 (229.39 KB) | In vitro Potency Testing of Leptospira interrogans serogroup canicola Bacterins | May 28, 2023 |
| 626 (216.91 KB) | In vitro Potency Testing of Leptospira kirschneri serogroup grippotyphosa Bacterins | May 28, 2023 |
| 627 (233.25 KB) | In vitro Potency Testing of Leptospira interrogans serogroup icterohaemorrhagiae Bacterins | May 28, 2023 |
| 630 (924.18 KB) | Potency Testing of Fowl Cholera (Pasteurella multocida) Bacterins, Type 4 | June 17, 2022 |
| 631 (933.65 KB) | Potency Testing of Salmonella typhimurium Bacterins | June 17, 2022 |
| 632 (936.52 KB) | Potency Testing of Salmonella choler aesuis Bacterins | June 17, 2022 |
| 633 (968.02 KB) | Potency Testing of Salmonella dublin Bacterins | June 17, 2022 |
| 634 (172.1 KB) | Potency Testing of Pasteurella multocida Bacterins of Bovine Origin | December 18, 2017 |
| 635 (217.46 KB) | Potency Testing of Pasteurella multocida Bacterins of Porcine Origin | December 18, 2017 |
| 636 (1.01 MB) | Evaluation of Tuberculin Purified Protein Derivative (PPD) | June 17, 2022 |
| ID | Title | Date |
|---|---|---|
| 801 (183.67 KB) | Examination of Porcine Cells for Porcine Parvovirus Contamination | September 1, 2023 |
| 802 (200.28 KB) | Supplemental Assay Method for Identification of Vaccine Strains of Newcastle Disease Virus | September 1, 2023 |
| 803 (159.05 KB) | Examination of Trypsin Solutions for Porcine Parvovirus Contamination | November 28, 2014 |
| ID | Title | Date |
|---|---|---|
| 900 (256.86 KB) | Testing Growth Promoting Qualities of Fluid Thioglycollate Medium and Soybean-Casein Digest Medium using Bacillus subtilis Spores and Issatchenkia orientalis as the Indicator Organisms | January 5, 2024 |
| 901 (231.87 KB) | Testing Growth-Promoting Qualities of Fluid Thioglycollate Medium with Beef Extract Using Clostridium chauvoei Spores as the Indicator Organism | January 5, 2024 |
| 902 (225 KB) | Testing Growth-Promoting Qualities of Brain Heart Infusion Agar using Bacillus subtilis Spores and Issatchenkia orientalis as Indicator Organisms | January 5, 2024 |
| 903 (210.16 KB) | Testing for Preservative Interference with Sterility Tests | January 3, 2022 |
| 905 (227.51 KB) | Potency of Live Avirulent Mannheimia haemolytica Vaccine | January 3, 2022 |
| 906 (252.52 KB) | Supplemental Assay Method for Sterility Testing of Preparations Other Than Live Vaccines | January 25, 2024 |
| 908 (252.27 KB) | Sterility Testing of Live Viral Vaccines and Master Seed Virus Samples | May 12, 2023 |
| 909 (230.64 KB) | Sterility Testing of Live Viral Vaccines of Chicken Embryo Origin Recommended for Nonparenteral Injection | May 12, 2023 |
| 910 (248.48 KB) | Detection of Mycoplasma Contamination | February 21, 2023 |
| 911 (250.03 KB) | Detection of Salmonella in Antibody Products | April 27, 2023 |
| 912 (203.68 KB) | Determination of Total Bacterial Count in Antibody Products | February 21, 2023 |
| 913 (230.76 KB) | Detection of Coliforms in Antibody Products | April 27, 2023 |
| 914 (193.99 KB) | Detection of Fungi in Antibody Products | May 15, 2023 |
| 918 (212.26 KB) | Bacterial Count of Pasteurella multocida, Avian Isolates, Vaccines | February 21, 2023 |
| 928 (227.99 KB) | Detection of Extraneous Bacteria and Fungi in Live Bacterial Vaccines and Master Seed Bacteria Samples | July 26, 2018 |